Restriction or amplification of wobble recognition: the structure of 2-thio-5-methylaminomethyluridine and the interaction of odd uridines with the anticodon loop backbone.
نویسندگان
چکیده
Physical and coding properties of poly (5-aminouridylic acid) and of 5-aminouridine-containing trinucleotides. Crystal structure and conformation of 3-deaza-4-deoxyuridine. 5-Substituents in the uridine moiety and their effect on the conformation of ApU type dinucleoside phosphates. Restriction or amplification of wobble recognition: The structure of 2-thio-5-methylaminomethyluridine and the interaction of odd uridines with the anticodon loop backbone. Crystal and molecular structure of 2-thio-5-carboxymethyluridine and its methyl ester: Helix terminator nucleosides in the first position of some anticodons. 5-Methoxyuridine: The influence of 5-substituents on the keto-enol tautomerism of the 4-carbonyl group. Crystal structure and conformation of 2-thio-5-methylaminomethyluridine dihydrate. Physical and coding properties of poly (5-methoxy-uridylic) acid.
منابع مشابه
Accurate translation of the genetic code depends on tRNA modified nucleosides.
Transfer RNA molecules translate the genetic code by recognizing cognate mRNA codons during protein synthesis. The anticodon wobble at position 34 and the nucleotide immediately 3' to the anticodon triplet at position 37 display a large diversity of modified nucleosides in the tRNAs of all organisms. We show that tRNA species translating 2-fold degenerate codons require a modified U(34) to enab...
متن کاملThe role of modifications in codon discrimination by tRNAUUU
The natural modification of specific nucleosides in many tRNAs is essential during decoding of mRNA by the ribosome. For example, tRNAUUU requires the modification N6-threonylcarbamoyladenosine at position 37 (t6A37), adjacent and 3′ to the anticodon, to bind AAA in the A site of the ribosomal 30S subunit. Moreover, it can only bind both AAA and AAG lysine codons when doubly modified with t6A37...
متن کاملLoss of Anticodon Wobble Uridine Modifications Affects tRNALys Function and Protein Levels in Saccharomyces cerevisiae
In eukaryotes, wobble uridines in the anticodons of tRNA(Lys)UUU, tRNA(Glu)UUC and tRNA(Gln)UUG are modified to 5-methoxy-carbonyl-methyl-2-thio-uridine (mcm5s2U). While mutations in subunits of the Elongator complex (Elp1-Elp6), which disable mcm5 side chain formation, or removal of components of the thiolation pathway (Ncs2/Ncs6, Urm1, Uba4) are individually tolerated, the combination of both...
متن کاملModified uridines with C5-methylene substituents at the first position of the tRNA anticodon stabilize U.G wobble pairing during decoding.
Post-transcriptional modifications at the first (wobble) position of the tRNA anticodon participate in precise decoding of the genetic code. To decode codons that end in a purine (R) (i.e. NNR), tRNAs frequently utilize 5-methyluridine derivatives (xm(5)U) at the wobble position. However, the functional properties of the C5-substituents of xm(5)U in codon recognition remain elusive. We previous...
متن کاملStructure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination.
tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- FEBS letters
دوره 94 2 شماره
صفحات -
تاریخ انتشار 1978